Biosynthesis of monoterpene hydrocarbons from [1-3H]neryl pyrophosphate and [1-3H]geranyl pyrophosphate by soluble enzymes from Citrus limonum
Identifieur interne : 004924 ( Main/Exploration ); précédent : 004923; suivant : 004925Biosynthesis of monoterpene hydrocarbons from [1-3H]neryl pyrophosphate and [1-3H]geranyl pyrophosphate by soluble enzymes from Citrus limonum
Auteurs : Liliana Chayet [Chili] ; Cecilia Rojas [Chili] ; E. Cardemil [Chili] ; Ana María Jabalquinto [Chili] ; R. Vicu A [Chili] ; O. Cori [Chili]Source :
- Archives of Biochemistry and Biophysics [ 0003-9861 ] ; 1977.
Abstract
A soluble enzyme preparation from the flavedo of Citrus limonum transforms [1-3H1]neryl pyrophosphate or [1-3H1]geranyl pyrophosphate into β-pinene, sabinene, α-pinene, and limonene. The enzyme has been partially purified and stabilized by precipitation with polyethyleneglycol. The enzymic cyclization requires the presence of Mn2+, which cannot be replaced with Mg2+. The addition of reagents containing sulfhydryl groups is essential for optimal activity. Allylic C10 monophosphates do not act as substrates, but they inhibit hydrocarbon formation. Inorganic pyrophosphate has a similar inhibitory effect. No interconversion of neryl and geranyl pyrophosphate has been observed. Possible pathways for the enzymic cyclization reactions are proposed.
Url:
DOI: 10.1016/0003-9861(77)90044-3
Affiliations:
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Vicu A</name></author><author><name sortKey="Cori, O" sort="Cori, O" uniqKey="Cori O" first="O." last="Cori">O. 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Cardemil</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Laboratory of General Biochemistry, Faculty of Chemical Sciences, Universidad de Chile, Casilla 233, Santiago 1</wicri:regionArea><wicri:noRegion>Santiago 1</wicri:noRegion></affiliation></author><author><name sortKey="Jabalquinto, Ana Maria" sort="Jabalquinto, Ana Maria" uniqKey="Jabalquinto A" first="Ana María" last="Jabalquinto">Ana María Jabalquinto</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Laboratory of General Biochemistry, Faculty of Chemical Sciences, Universidad de Chile, Casilla 233, Santiago 1</wicri:regionArea><wicri:noRegion>Santiago 1</wicri:noRegion></affiliation></author><author><name sortKey="Vicu A, R" sort="Vicu A, R" uniqKey="Vicu A R" first="R." last="Vicu A">R. 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Cori</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Laboratory of General Biochemistry, Faculty of Chemical Sciences, Universidad de Chile, Casilla 233, Santiago 1</wicri:regionArea><wicri:noRegion>Santiago 1</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Biochemistry and Biophysics</title><title level="j" type="abbrev">YABBI</title><idno type="ISSN">0003-9861</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1977">1977</date><biblScope unit="volume">180</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="318">318</biblScope><biblScope unit="page" to="327">327</biblScope></imprint><idno type="ISSN">0003-9861</idno></series><idno type="istex">C4A3FE00EC9EB3B7486725FAF6ADF7F04C812724</idno><idno type="DOI">10.1016/0003-9861(77)90044-3</idno><idno type="PII">0003-9861(77)90044-3</idno><idno type="ArticleID">77900443</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9861</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A soluble enzyme preparation from the flavedo of Citrus limonum transforms [1-3H1]neryl pyrophosphate or [1-3H1]geranyl pyrophosphate into β-pinene, sabinene, α-pinene, and limonene. The enzyme has been partially purified and stabilized by precipitation with polyethyleneglycol. The enzymic cyclization requires the presence of Mn2+, which cannot be replaced with Mg2+. The addition of reagents containing sulfhydryl groups is essential for optimal activity. Allylic C10 monophosphates do not act as substrates, but they inhibit hydrocarbon formation. Inorganic pyrophosphate has a similar inhibitory effect. No interconversion of neryl and geranyl pyrophosphate has been observed. Possible pathways for the enzymic cyclization reactions are proposed.</div></front></TEI><affiliations><list><country><li>Chili</li></country></list><tree><country name="Chili"><noRegion><name sortKey="Chayet, Liliana" sort="Chayet, Liliana" uniqKey="Chayet L" first="Liliana" last="Chayet">Liliana Chayet</name></noRegion><name sortKey="Cardemil, E" sort="Cardemil, E" uniqKey="Cardemil E" first="E." last="Cardemil">E. Cardemil</name><name sortKey="Cori, O" sort="Cori, O" uniqKey="Cori O" first="O." last="Cori">O. Cori</name><name sortKey="Jabalquinto, Ana Maria" sort="Jabalquinto, Ana Maria" uniqKey="Jabalquinto A" first="Ana María" last="Jabalquinto">Ana María Jabalquinto</name><name sortKey="Rojas, Cecilia" sort="Rojas, Cecilia" uniqKey="Rojas C" first="Cecilia" last="Rojas">Cecilia Rojas</name><name sortKey="Vicu A, R" sort="Vicu A, R" uniqKey="Vicu A R" first="R." last="Vicu A">R. Vicu A</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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